The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. STANDARD . Read & analyse. At Cisbio we provide microplates that help you discover better. PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. PURCHASE SEPARATELY . Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Figure 3. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. For more information about HTRF. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER. Homogeneous Time-Resolved Fluorescence (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based) • Kinases and ATPases • Cytokine Assays TUNE Multi-Mode • cAMP Quantitation • Cell Migration • Cell Viability and Proliferation • Cytotoxicity • DNA/RNA Quantitation (Fluorescent) Homogeneous Time-Resolved Fluorescence (HTRF) . Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Make sure the setup for Tb Cryptate is used. This protocol, HTS designed, allows miniaturization while. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. HTRF is compatible with a large range of available microplate readers. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Read & analyse. The FLUOstar Omega is the ideal plate reader for life science applications. These plates can be read on all HTRF certified readers, thus making their implementation in. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within theassay linear range 6 Add 4 μL of premixed antibody solutions (vol/vol) prepared in the detection buffer. FOR HTRF CERTIFIED READER. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. HTRF certified . Make sure the plate reader is turnedon and then open the MikroWin software on the computer. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Both Microplate Optimization and Read Height Adjustment were. Read & analyse. 3. 10 µL Standard or Sample. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . HTRF is compatible with a large range of available microplate readers. Incubate 4h at room temperature. SV detection plate. Unlike standard ELISA plate readers, MSD. FOR HTRF CERTIFIED READER For. FOR HTRF CERTIFIED READER. certified for use with HTRF. The signals are produced by, converted by, or transmitted through the. Incubate overnight at RT. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. CLARIOstar is equipped with a specific optical device which enables the measurement of. com HTRF Microplate Readers. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. It measures at any wavelength, with top and bottom reading for most standard technologies. no. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Make sure the setup for Tb Cryptate is used. For more information. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Plate 25 μL of cells in. 2. Endpoint mode experiments measure each well of the microplate only once. Lysates are then transferred to the assay plate for the detection of Total-eNOS by HTRF. Assay detection requires a microplate reader with. Plate 25 μL of cells in. Plate 25 μL of cells in. Highly compatible plates for HTRF. Figure 1: Principle of HTRF Prostaglandin E2 competitive assay. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Step 1 Step 2 Step 3 Step 4 Step 5. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. The plate was covered and incubated for one hour at room temperature. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. HTRF 96 TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: 3h * * Depending on cell lines used, volume of lysis should be optimized. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. The assay was prepared in both white and black plates and incubated >18 hours. + 4 µL HTRF ® pre-mixed antibo dies 2. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. This protocol gives the cells viability and confluence to be monitored. Its. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Read & analyse. For more. It also integrates with the powerful SoftMax Pro Software for. For more information about HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. * For HTRF microplate recommendations, please visit . 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. Incubate 4h at RT. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF® 96-well low volume plate TECHNOLOGY ALL THE BENEFITS OF HTRF WITH THE EASE OF USE OF AN ELISA PLATE!. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. * For HTRF microplate recommendations, please visit . At Cisbio we provide microplates that help you discover better. • HTRF®-Certified Reader. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Bench top solutions for cell based assays. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. COMPONENTS ; STORAGE . NC1286225. Interaction with the industry and research communities is essential for innovating! For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Assay setup for a 384-well low-volume plate. Manufacturer. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. Plate 25 μL of cells in. Incubate overnight at RT. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Assay plate setup. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. This protocol gives the cells viability and confluence to be monitored. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For a list of HTRF-compatible readers and set-up recommendations, please visit . 5 nm filter (PerkinElmer 2100-5110) were used. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization. HTRF plate reader. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Highly compatible plates for HTRF. Lysates are then transferred to the assay plate for the detection of total-CDK4 by HTRF. 47 seconds for a 1536-well plate. For HTRF readings, a 620/40 nm filter (PerkinElmer 2100-5760) and a 665/7. Incubate overnight at RT. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. For more information. 5. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. Read other about this measurement approach here. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Make sure the setup for Tb Cryptate is used. Lyse cells Read on an HTRF compatible reader . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. An. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. FOR HTRF CERTIFIED READER. Customer Transformation Hub. These plates can be read on all HTRF certified readers, thus making their implementation in. Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. Cells in each image are identified by SoftMax Pro Software and cell-by-cellHTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. 96-well culture plate 384SV detection plate- Add . HTRF reagent concentrations have been set for optimal assay performances. Other technologies such as imaging. The measurement of HTRF assays is predominantly performed on microplate readers. For more information about HTRF ®Highly compatible plates for HTRF. FOR HTRF CERTIFIED READER For more. For more information about HTRF. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. FOR HTRF CERTIFIED READER For. Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. Partnerships for delivering turn-key solutions. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. No washing steps are required. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. FlexStation® 3 Multi-Mode Microplate Reader. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Seal the plate and incubate 3 hours at RT . The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. The VICTOR Nivo. 3 and higher include optimized reader settings, as well as data analysis, for faster results. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. At its core, Synergy H1 is a monochromator-based multi-mode microplate reader with a quadruple grating architecture for robust performance and flexibility. These are the different kinetic modes on BMG LABTECH microplate readers. FOR HTRF CERTIFIED. For instance, when assay volumes are. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. . 68. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. FOR HTRF CERTIFIED READER. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. This protocol. As shown in Fig. 5 μL of Binding buffer 2X. Incubate 2 h at RT. The donor and acceptor can be used to label a variety of biomolecules, for. These plates can be read on all HTRF certified readers, thus making their implementation in. (donor) of compounds are measured on an HTRF-compatible reader. In this case the Z´(pronounced Z prime) value comes to mind. FOR HTRF CERTIFIED. The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. The ratio between control lysate signal / non-specific signal should be greater than 2. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. Eu cryptate emission level). Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. As shown in Fig. Add. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. For instance, when assay volumes are. The plate was covered and incubated for two hours at room temperature. MATERIALS PROVIDED: KIT . Phospho-Rb (Ser780) two-plate assay protocol. * For HTRF microplate recommendations, please visit binding of the fluorescent ligand to the receptor, TR-FRET occurs and is measured with an HTRF-certified microplate reader. If not, our scientific team will help you choose the instrument that best suits your needs and budget. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. FOR HTRF CERTIFIED READER For more information about HTRF. Revvity. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. NC1656382. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. This protocol, HTS designed, allows miniaturization while. FOR HTRF CERTIFIED READER For more information about HTRF. For the standard curve described, all wells of the microplate are read. For more ® information about HTRF compatible readers and for set-up recommendations. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. This protocol gives the cells viability and confluence to be monitored. FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. Read & analyse. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Lysates are then transferred to the assay plate for the detection of total-BTK by HTRF. This protocol, HTS designed, allows miniaturization while. Simply place the module into one of the five module bays and read the assay. acceptor reagent. It accepts all types of microtitration plate. Conclusion. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Use simpler, faster protocols. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersONE-PLATE ASSAY PROTOCOL: Small volume detection plate. + 4 µL HTRF ® pre-mixed antibo dies 2. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. FOR HTRF CERTIFIED READER . Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Incubate 24 hours at RT. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. They can be used in a no-transfer protocol with suspension cells or with. FOR HTRF CERTIFIED READER. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. If not, our scientific team will help you choose the instrument that best suits your needs and budget. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Plate 25 μL of cells in. + 4 µL HTRF pre-mixed antibodies. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. FOR HTRF CERTIFIED READER. 4 mg/ml. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. Read & analyse. Plate 25 μL of cells in. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. • HTRF®-Certified Reader **. Plate 25 μL of cells in. This protocol, HTS designed, allows miniaturization while. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Dual-Luciferase® (DLR™) gene assays. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. We would like to show you a description here but the site won’t allow us. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Cover the plate with a plate sealer. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Results for HTRF Reader Control Kit with black and white microplates. Plate 25 μL of cells in. The VICTOR Nivo is a HTRF certified microplate reader. 41 mg/ml) was obtained from Thermo Fisher Scientific. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. Incubate overnight at RT. FOR HTRF CERTIFIED READER. Make sure the setup for Tb Cryptate is used. If not, our scientific team will help you choose the instrument that best suits your needs and budget. COMPONENTS ; STORAGE . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. PROTOCOL AT A GLANCE. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. The laser beam passes through the sample well. HTRF is a proprietary time-resolved fluorescence technology that overcomes Results summary for HTRF IP1 standard curve. Excitation lasers significantly improve performance and lower. HTRF is a FRET-based technology that emerged from the Jean-Marie Lehn ’s Nobel prize-winning work on rare earth complexes. Download Setup. Make sure the setup for Eu Cryptate is used. Spark - Technical Notes. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. Microplate reader assays requiring infrared (IR) or. The HTRF ratio obtained represents the total binding. Quantify up to 10 analytes per well. Alpha Technology: The VICTOR Nivo system. Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. Remove the plate seal and read on an HTRF compatible reader. For more. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. The PHERAstar ® FSX high-throughput screening microplate reader uses unique, easy-to-configure optic modules. HTRF-specific. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. LANCE ® Ultra DELFIA ® Certified HTRF Readers and Setups Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. → Cover the plate with a plate sealer. -Certified Reader. It can also be further streamlinedFOR HTRF CERTIFIED READER. MUTANT HTT STANDARD WORKING SOLUTIONSLysis buffer 4X for two-plate assay protocol on suspension cells Determine the amount of lysis buffer needed for the experiment. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Make sure the setup for Eu 3+ Cryptate is used. Revvity. Make sure the setup for Eu 3+ Cryptate is used. Lysates are then transferred to the assay plate for the detection of Total H2AX by HTRF ® reagents. 1. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. View Brochure. FOR HTRF CERTIFIED READER. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 8 µL cells. 1) . Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. The donor and acceptor can be used to label a variety of biomolecules, for applications. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The optical systems use two scanning All the benefits of HTRF with ELISA plate ease of use. 3. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). Make sure the setup for Tb Cryptate is used. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. It can also be further streamlined to a one-. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). 5 hours before 3 more washing steps. These plates can be read on all HTRF certified readers, thus making their implementation in.